Evaluation of a new 5'-nuclease real-time PCR assay targeting …
A new 5'-nuclease real-time PCR assay that targets the 300-fold repeated AF146527 DNA sequence (TaqMan-AF-PCR) has been developed and its performance for diagnosis of …
Multicenter evaluation of the Toxoplasma gondii Real-TM …
Mar 12, 2024 · Toxoplasma gondii Real-TM (Sacace, Como, Italy) is a real-time PCR kit, targeting the Rep529 non-coding repetitive DNA sequence, for T. gondii DNA detection in clinical …
Molecular Diagnosis of Toxoplasmosis - ScienceDirect
Jun 1, 2022 · Real-time PCR plays a crucial role in the diagnosis of toxoplasmosis. In this multicenter study, the Toxoplasma RealCycler Universal assay was assessed for the …
Is Real-Time PCR Targeting Rep 529 Suitable for Diagnosis of ...
Multicentric comparative assessment of the bio-evolution Toxoplasma gondii detection kit with eight laboratory-developed PCR assays for molecular diagnosis of congenital toxoplasmosis.
Serious complications may occur in utero, neonatally, or years after birth when congenital toxoplasmosis is acquired during pregnancy. Real-time PCR provides a rapid and sensitive …
Herein, we determine the analytical characteristics of a single-reaction, internally controlled, dual-target, real-time T. gondii PCR and evaluate the clinical performance of this assay in …
Real-time PCR targeting a 529-bp repeat element for diagnosis …
This study demonstrates the higher sensitivity of the 5'-nuclease real-time PCR assay developed for the AF146527 DNA sequence and confirms the interest of using this highly repeated target …
Evaluation of Toxoplasma ELITe MGB Real-Time PCR Assay for …
In this multicenter study, we evaluated the performances of the Toxoplasma ELITe MGB kit (Elitech, Puteaux, France), a rep529 -targeting assay, using serial dilutions of calibrated …
Genotype distribution and risk factors of Toxoplasma gondii …
6 days ago · PCR amplification of Toxoplasma gondii B1 Gene Nested PCR targeting the Toxoplasma gondii B1 gene was performed to detect parasite DNA [22]. The primary PCR …
Dual-target, real-time PCR for the diagnosis of intraocular
Herein, we determine the analytical characteristics of a single-reaction, internally controlled, dual-target, real-time T. gondii PCR and evaluate the clinical performance of this assay in …